个人简介:
温大兴,博士,副教授,硕士生导师。2017年6月毕业于365be体膏官网,获农学博士学位;2017年8月至2019年8月,在365be体膏官网生物学博士后科研流动站从事博士后研究;2019年9月至今,在365be体膏官网工作。主持国家自然科学基金面上项目1项、山东省自然科学基金青年基金1项,参与国家级和省部级项目3项,指导国家级SRT项目1项。在Food Chemistry、Plant Methods、International Journal of Molecular Sciences等期刊发表论文10余篇,授权发明专利1项。主讲课程:《种子检验学》(山东省一流本科课程)、《种子实验技术》、《作物种子学教学实习》。
主要研究方向:
1. 玉米种子快速萌发出苗关键基因的克隆和调控机理解析
通过GWAS、BSA-seq、QTL定位等技术,挖掘玉米种子快速萌发出苗关键基因并验证其功能,保障玉米出苗快速、整齐,为高产稳产打好基础。
2. 高活力种子的调控机理解析
通过转录组学、蛋白质组学、代谢组学等技术,研究影响小麦和玉米高活力种子形成的因素,揭示高活力种子的调控机理,为高质量种子 生产提供依据。
3. 研发种子检验新技术
改进现有种子检验方法的不足、创建新的种子检验方法,提高种子检验效率。目前,已经创建了种子纯度快速分子检测技术、种子活力快速无损检测技术。
近年来主持项目:
1. 国家自然科学基金面上项目,ZmML3基因调控玉米中胚轴伸长的分子机制,2023-01至2026-12,主持。
2. 山东省自然科学基金青年基金,玉米中胚轴伸长基因qML3的克隆和功能研究,2022-01至2024-12,主持。
3. 山东省重点研发计划,玉米精准定向分子育种技术体系构建与应用,2021-12至2025-06,参与。
4. 国家自然科学基金面上项目,高活力种子萌发期抗病菌侵染的机理研究:以玉米和禾谷镰刀菌为例,2020-01至2023-12,参与。
5. 山东省农业良种工程项目,2019LZGC002-1,粮饲兼用型宜机收玉米突破性新品种选育,2019-08至2022-08,参与。
近年来主要代表论文:
1. Jian Liu#, Daxing Wen#, Xianliang Song, Peisen Su, Jianfeng Lou, Danqing Yao, Chunqing Zhang*. Evolution and natural selection of ribosome-inactivating proteins in bacteria, fungi, and plants. International Journal of Biological Macromolecules, 2023, 248: 125929.
2. Aiju Meng#, Daxing Wen#, Chunqing Zhang*. Dynamic Changes in Seed Germination under Low-Temperature Stress in Maize. International Journal of Molecular Sciences, 2022, 23(10): 5495.
3. Aiju Meng#, Daxing Wen#, Chunqing Zhang*. Maize Seed Germination under Low-Temperature Stress Impacts Seedling Growth under Normal Temperature by Modulating Photosynthesis and Antioxidant Metabolism. Frontiers in Plant Science, 2022, 13: 843033.
4. Min Li#, Daxing Wen#, Qingqing Sun#, Chenglai Wu, Yan Li, Chunqing Zhang*. Factors influencing seed reserve utilization during seedling establishment in maize inbred lines. Journal of Integrative Agriculture, 2022, 21(3): 677-684.
5. Liuyong Xie#, Daxing Wen#, Chenglai Wu, Chunqing Zhang*. Transcriptome analysis reveals the mechanism of internode development affecting maize stalk strength. BMC Plant Biology, 2022, 22: 49.
6. Daxing Wen, Haicheng Xu, Mingrong He, Chunqing Zhang*. Proteomic analysis of wheat seeds produced under different nitrogen levels before and after germination. Food Chemistry, 2021, 340: 127937.
7. Daxing Wen#, Yan Li#, Lifeng He, Chunqing Zhang*. Transcriptome analysis reveals the mechanism by which spraying diethyl aminoethyl hexanoate after anthesis regulates wheat grain filling. BMC Plant Biology, 2019, 19: 327.
8. Daxing Wen, Haicheng Xu, Liuyong Xie, Mingrong He, Hongcun Hou, Chenglai Wu, Yan Li, Chunqing Zhang*. Effects of nitrogen level during seed production on wheat seed vigor and seedling establishment at the transcriptome level. International Journal of Molecular Sciences, 2018, 19(11): 3417.
9. Daxing Wen, Hongcun Hou, Aiju Meng, Jie Meng, Liuyong Xie, Chunqing Zhang*. Rapid evaluation of seed vigor by the absolute content of protein in seed within the same crop. Scientific Reports, 2018, 8: 5569.
10. Daxing Wen, Haicheng Xu, Liuyong Xie, Mingrong He, Hongcun Hou, Chunqing Zhang*. A loose endosperm structure of wheat seed produced under low nitrogen level promotes early germination by accelerating water uptake. Scientific Reports, 2017, 7: 3116.
11. Jianpeng Zheng#, Daxing Wen#, Hongchun Zhao, Chunqing Zhang*. Acetic acid urea-polyacrylamide gel electrophoresis: a rapid method for testing the genetic purity of sunflower seeds. Quality Assurance and Safety of Crops & Foods, 2017, 9(1): 41-46.
12. Chunwei Wang, Daxing Wen, Aiqing Sun, Xiaoyu Han, Jiedao Zhang, Zhenlin Wang, Yanping Yin*. Differential activity and expression of antioxidant enzymes and alteration in osmolyte accumulation under high temperature stress in wheat seedlings. Journal of Cereal Science, 2014, 60(3): 653-659.
13. Daxing Wen, Chunqing Zhang*. Universal Multiplex PCR: a novel method of simultaneous amplification of multiple DNA fragments. Plant Methods, 2012, 8:32.
联系方式:
山东省泰安市岱宗大街61号,365be体膏官网种子科学与工程系,271018
电子邮箱: dxwen@sdau.edu.cn